携带NDV HN基因疫苗的重组减毒鸡白痢沙门菌口服免疫原性研究

doi:10.11843/j.issn.0366-6964.2016.10.017

Abstract

Abstract:

To study the oral immunogenicity of attenuated Salmonella pullorum，carried HN (haemagglutinin-neuraminidase) gene vaccine of Newcastle disease virus (NDV).In this study，the recombinant plasmid pcDNA3-HN was finally electro-transformed into attenuated Salmonella pullorum ΔcrpC79-13 to construct the recombinant strain ΔcrpC79-13 (pcDNA3-HN).Then the 7-day-old chickens were oral inoculated with 1×10⁹CFU recombinant strain ΔcrpC79-13 (pcDNA3-HN)，and the Δcrp C79-13 (pcDNA3)，PBS，NDV Ⅳvaccine were act as the control group.Then the dynamic level of HI antibody and intestinal mucosal IgA antibody against NDV were determined at 7，14，21，28，35 days post vaccination.At the same time，the level of peripheral blood lymphocyte proliferation of immune chicken and the challenge protection efficiency were determined.The results showed that the recombinant vaccine strains Δcrp C79-13 (pcDNA3-HN) carrying NDV HN gene vaccine was constructed successfully，which could induce HI antibody against NDV at 14-35 d after immunization and reach the highest value at 21 d after immunization.The content of intestinal mucosal IgA antibody in Δcrp (C79-13 pcDNA3-HN) group was slightly higher than that in Δcrp C79-13 (pcDNA3) group，but the difference was not significant (P> 0.05).The peripheral blood lymphocyte stimulation index of Δcrp (C79-13 pcDNA3-HN) group was significantly higher than that of the PBS control group (P<0.01)，and was slightly higher than that of the ΔcrpC79-13 (pCDNA3) group （P＜0.05) at 28 d after immunization.The immunity protection rate against strong virus was 67%（10/15） in experiment group with 10³EID₅₀NDV F₄₈E₉，the protection rate was 100% which challenged with 10⁸ CFU Salmonella pullorum C79-13 strain.The results showed that the recombinant attenuated Salmonella pullorum ΔcrpC79-13 (pcDNA3-HN) strains have good oral immunogenicity.

CLC Number:

S852.52

DING Ke，YU Zu-hua，LI Meng，YU Chuan，SHANG Ke，CHENG Xiang-chao，CHEN Gui-hua，LIAO Cheng-shui, JIA Yan-yan, WANG Yang, ZHANG Chun-jie. Oral Immunogenicity of an Attenuated Salmonella pullorum Harbouring HN Gene Vaccine of Newcastle Disease Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2081-2088.

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Oral Immunogenicity of an Attenuated Salmonella pullorum Harbouring HN Gene Vaccine of Newcastle Disease Virus

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